In Perfect condition Key applications: ChIP sequencing Exome sequencing Gene expression sequencing De novo sequencing Small RNA sequencing Whole transcriptome sequencing
Areas of interest: Agricultural research Stem cell research Cancer research Epigenomics Ancient DNA genomics Metagenomics
Chip compatibility Ion PI v3 Chip
60–80M reads ~2.5 hours run time
Ion Proton System performance specifications with Ion PI Chip
ThroughputUp to 15 Gb Read lengthUp to 200-base fragment readsNumber of reads passing filter60-80 million reads passing filterSequencing run time2.5 hoursBarcoding solutions384 barcodes supported by Torrent Suite Software 96 off-the-shelf barcodes for DNA 16 off-the-shelf barcodes for RNASoftware solutions Torrent Suite Software utilizing Torrent Browser including TMAP alignment and Torrent Variant Caller for germline or somatic mutation detection.
Torrent Browser (included) offers users remote web access to instrument status monitoring, run quality reports, individual data files, and extensible plug-ins for application-specific analysis. Plugins available for download from the Torrent Browser Plugin Store for analysis of genome sequencing, targeted sequencing, variant analysis and annotation, microbial sequencing, transfer to 3rd party bioinformatics packages, and more. Data can optionally flow into Ion Reporter Software for controlled analysis, annotation, and reporting of variants. Ion Reporter Software provides application specific workflows to support single, trio and paired tumor normal analysis. Data formatsIndustry standard FASTQ, SFF, BAM and VCF format outputs
Specifications for the Ion Proton Sequencer
Working environment (for indoor use only)Temperature: 68-77° F (20-25° C) Humidity: 40-60%, noncondensing Altitude: <6,500 ft (2,000 m) Clearances:
12 in (30.5 cm) in rear 4 in (10 cm) on left side 4 in (10cm) on right side 4 in (10 cm) from front edge of bench to sequencer bezel 36 in (90 cm) aisle in front of bench for operator access
Optional rack mounting with two Ion Proton Sequencers per rack†Gas SupplyConnection: 0.25 in push-to-connect fitting Pressure: 30 psi Composition: nitrogen (grade 4.8, 99.998% or better)Other connectionsEthernet: 1 GigE USB: 2x USB 2.0 PowerVoltage: 100 V (min) to 240 V (max) Current: 14 A (max) Frequencing: 50/60 Hz Power Draw: 1,350 WDimensionsWidth: 21.3 in/54.2 cm Depth: 30.5 in/77.5 cm Height: 18.7in/47.4 cmWeightCrated for shipment: 200 lb/90.7 kg Free-standing: 130
34 x 42 x 38 IN - 160 LBS
863.60 x 1066.80 x 965.20 mm - 72.58 kg
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