- Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader
- Molecular Devices SpectraTest FL1 Validation Plate
- Molecular Devices SpctraTest ABS1 Absorbance Validation Plate
- Cables and connectors
The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. These robust readers have been placed in labs from Antarctica to the International Space Station. With optimized reagents, validation tools, and industry-leading SoftMax® Pro Software, they provide consistent performance.
The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. This application note describes how to run IMAP fluorescence polarization (FP) kinase assays and calibration curves with green- and red-labeled fluorescent substrates using the SpectraMax M5 microplate reader and SoftMax® Pro Software. Enzyme dilution curves were performed for Lck, a tyrosine kinase with a key role in T-cell signaling, and Akt1/ PKBa, a serine-threonine kinase involved in phosphatidylinositol 3-kinase signaling and cell survival. Inhibition of Akt1/PKBa by staurosporine was also assayed. High Z’ factor values are obtained with both FAMand TAMRA-labeled peptide substrates, with data comparable to those obtained on the filter-based Analyst® HT Multi-Mode Microplate Reader.