Please note: This equipment is currently in storage and not located at its original location. To see buyer requirements click here To See The Sales Agreement Click Here Molecular Devices Plate Reader Spectra Max M2
SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. Endpoint, kinetic, spectrum and area-well scanning read types and PathCheck® allow homogeneous and heterogeneous microplate assays to be performed in this flexible system. The FI and Abs microplate reader with built-in cuvette port allows easy conversion and optimization of very-low-throughput assays into medium-high-throughput microplate assays. The SpectraMax M2 allows faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command
MFG. Specifications
Absorbance Photometric Performance
Wavelength range: 200-1000 nm
Wavelength selection: Monochromator, tunable in 1.0 nm increments
Wavelength bandwidth: ≤ 4.0 nm
Wavelength accuracy: ±2.0 nm
Wavelength repeatability: ±0.2 nm
Photometric range: 0-4.0 OD
Photometric resolution: 0.001 OD
Photometric accuracy (microplate): < ±0.006 OD ±1.0%, 0-2 OD
Photometric accuracy (cuvette): < ±0.005 OD ±1.0%, 0-2 OD
Photometric precision: < ±0.003 OD ±1.0%, 0-2 OD
Baseline flatness: < 0.001 OD
Stray light: < 0.05% @ 230 nm
Fluorescence Photometric Performance (M2)
Dual monochromators: 1 nm increments
EX 250-850 nm
EM 360-850 nm
Bandwidth (EX, EM): 9, 9 nm
Detection limit: 3.0 fmol/well FITC 200 μL in 96 wells (signal 3X SD of baseline)
Fluorescence Photometric Performance (M2e)
Dual monochromators: 1 nm increments
EX 250-850 nm
EM 250-850 nm
Bandwidth (EX, EM): 9, 9 nm
Top-read detection limit: 3.0 fmol/well FITC 200 μL in 96 wells (signal 3X SD of baseline)
Bottom-read detection limit: 5.0 fmol/well FITC 200 μL in 96 wells (signal 3X SD of baseline)
Time-Resolved Fluorescence (Secondary Mode)
Wavelength range (M2): 360-850 nm
Wavelength range (M2e): 250-850 nm
Data collection: 50-1450 μsec., 200 μsec. increments
Sensitivity: 0.5 fmol/well Eu-chelate (obtained with DELFIA® reagent from Perkin Elmer by using a 384-well plate)
Luminescence (Secondary Mode)
Wavelength range (M2): 360-850 nm
Wavelength range (M2e): 250-850 nm
Detection limit: 10 amol/well alkaline phosphatase 200 μL/well (obtained with Emerald IITM reagent from Applied Biosystems)
General Photometric Performance
Plate formats: 6, 12, 24, 48, 96, 384 wells
Light source: Xenon flash lamp (1 joule/flash)
Detector: Photomultiplier (R-3896)
Read time* 96-well: Abs 18 sec., FI 15 sec.; 384-well: Abs 49 sec., FI 45 sec.
Shaker time: 0 to 999 seconds
Temp. control: 4°C above ambient to 45°C
Temp. uniformity: < 1°C at 37°C set point
Temp. accuracy: ±1°C at 37°C set point
* Measurement type may extend read time.
General Specifications
Dimensions (in.): 8.6 (H) x 22.8 (W) x 15 (D)
Dimensions (cm): 22 (H) x 58 (W) x 38 (D)
Weight: 35 lbs. (15.9 kg)
Power consumption: < 125 watts
Power source: 100-240 Vac, 3 A, 50/60 Hz
Plastic protctive cover
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